| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Fusobacterium necrophorum | TGCGGAAGACATTTCTCAGGA | TTCCCTCTCCTGCATGGAAAT | 59.38 | 59.07 | 247 | 128 |
100.00%
|
50.00%
|
| Primer 42 | Fusobacterium necrophorum | TGCGGAAGACATTTCTCAGGA | AATTCCCTCTCCTGCATGGAA | 59.38 | 59.07 | 249 | 128 |
100.00%
|
50.00%
|
| Primer 43 | Fusobacterium necrophorum | AGCACAGAGAGCGGAAGAA | TGCAGTCGAACCAAGGCA | 58.64 | 59.49 | 234 | 128 |
100.00%
|
50.00%
|
| Primer 44 | Fusobacterium necrophorum | AGAAGCACAGAGAGCGGAA | TGCAGTCGAACCAAGGCA | 58.64 | 59.49 | 237 | 128 |
100.00%
|
50.00%
|
| Primer 45 | Fusobacterium necrophorum | AAGCACAGAGAGCGGAAGAA | TGCAGTCGAACCAAGGCA | 59.32 | 59.49 | 235 | 128 |
100.00%
|
50.00%
|
| Primer 46 | Fusobacterium necrophorum | AAGAAGCACAGAGAGCGGAA | TGCAGTCGAACCAAGGCA | 59.32 | 59.49 | 238 | 128 |
100.00%
|
50.00%
|
| Primer 47 | Fusobacterium necrophorum | ATGATGACGTAGCGGAGGC | GTCTTCTTCTCCTGCCAGCT | 59.64 | 59.39 | 200 | 128 |
100.00%
|
50.00%
|
| Primer 48 | Fusobacterium necrophorum | AGGACGTGTTCTGGCGAA | TCAATGACAACGCCTCCCC | 58.87 | 60.00 | 201 | 128 |
100.00%
|
50.00%
|
| Primer 49 | Fusobacterium necrophorum | CTGGATGTCGGGTGTGGAA | CCAACTCCTTCACAGCTTGC | 59.32 | 59.40 | 152 | 128 |
100.00%
|
50.00%
|
| Primer 50 | Fusobacterium necrophorum | CAACACCGTTAGGAGGGCA | GGCACCGTCTAAGGTAACCC | 59.63 | 60.11 | 161 | 127 |
100.00%
|
50.00%
|